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43-277 (0.01ug/ml) staining of nuclear Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-277 (0.01ug/ml) staining of nuclear Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CDC6 Antibody

CATALOG NUMBER: 43-277

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: RKSQEITTNSEQRCP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in nuclear lysates of cell lines HeLa and Jurkat (calculated MW of 62.7kDa according to NP_001245.1). Recommended concentration: 0.01-0.03ug/ml.
Predicted Molecular Weight:
Approx 70kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CDC6
Additional Names:
CDC6, cell division cycle 6 homolog (S. cerevisiae), CDC18L, HsCDC18, HsCDC6, CDC18 (cell division cycle 18, S.pombe, homolog)-like, CDC6 cell division cycle 6 homolog, CDC6-related protein, cell division cycle 6 protein
Protein Accession Number:
NP_001245.1
Protein Gi Number:
50400620
Ncbi Gene Id Number:
990
Background Reference 1:
Liu L, Choi JH, Yim H, Choi JS, Park BD, Cho SJ, Lee SK, ATR (AT mutated Rad3 related) activity stabilizes Cdc6 and delays G2/M-phase entry during hydroxyurea-induced S-phase arrest of HeLa cells.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-277

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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