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Western blot analysis of CDCA8 in Rat kidney lysate with CDCA8 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of CDCA8 in rat kidney tissue with CDCA8 antibody at 20 μg/mL.
Western blot analysis of CDCA8 in Rat kidney lysate with CDCA8 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

CDCA8 Antibody

CATALOG NUMBER: 4751

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
CDCA8 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of the human CDCA8.
The immunogen is located within the first 50 amino acids of CDCA8.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
CDCA8 antibody can be used for detection of CDCA8 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1465 - Rat Kidney Tissue Lysate
Purification:
CDCA8 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CDCA8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CDCA8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDCA8
Additional Names:
CDCA8 Antibody: BOR, DasraB, MESRGP, BOREALIN, PESCRG3, Borealin, Cell division cycle-associated protein 8, hDasra-B
Protein Accession Number:
NP_060571
Protein Gi Number:
8922438
Ncbi Gene Id Number:
55143
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CDCA8 Antibody: CDCA8 is a component of a chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The chromosomal passenger complex, which includes Survivin, CDCA8, INCENP and Aurora-B, is known to play crucial roles during mitosis and cell division. It was found that CDCA8 interacting with Aurora-B, INCENP and Survivin, increases during G2/M phase and then reduces after exit from M phase. CDCA8 is cell cycle regulated, down-regulated in response to p53/Rb-signaling, and up-regulated in many types of cancerous tissues. In Drosophila cells, inactivation of CDCA8 results in polyploidy, delayed mitosis and abnormal tissue development, indicating its critical role for cell proliferation. Recent studies show that CDCA8 is essential for cell proliferation during early embryonic development, and its early embryonic lethality cannot be rescued by the loss of p53. Its aberrant expression is linked to a poor prognosis for gastric cancer.
Background Reference 1:
Gassmann R, Carvalho A, Hanzing AJ, et al. Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle. J. Cell Biol.2004; 166:179-91.
Background Reference 2:
Tani M, Okino N, Mitsutake S, et al. Molecular Cloning of the Full-length cDNA Encoding Mouse Neutral Ceramidase. J. Biol. Chem.2000; 275:11229-34.
Background Reference 3:
Hanson KK, Kelley AC, and Bienz M Loss of Drosophila borealin causes polyploidy, delayed apoptosis and abnormal tissue development. Development2005; 132:4777-87.
Background Reference 4:
Date DA, Jacob CJ, Bekier ME et al. Borealin is repressed in response to p53/Rb signaling. Cell Biol. Int.2007; 31:1470-81.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4751

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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