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45-031 (1ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-031 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-031 (1ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

CDCP1 Antibody

CATALOG NUMBER: 45-031

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-PLLNTQEPMEPAE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 90kDa band observed in Human Colon lysates (calculated MW of 92.3kDa according to NP_073753.3). Recommended concentration: 1-3ug/ml.An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize isoform 1 (NP_073753.3) only.
Positive Control 1:
Cat. No. 1320 - Human Colon Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CDCP1
Additional Names:
CDCP1, CUB domain containing protein 1 , HGNC:24357, CD318, SIMA135, TRASK , CUB domain-containing protein 1, transmembrane and associated with src kinases
Protein Accession Number:
NP_073753.3
Protein Gi Number:
30410805
Ncbi Gene Id Number:
64866
Background Reference 1:
Hooper JD, Zijlstra A, Aimes RT, Liang H, Claassen GF, Tarin D, Testa JE, Quigley JP Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen. Oncogene. 2003 Mar 27;22(12):1783-94.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-031

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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