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Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Immunofluorescence analysis of CDH4 Antibody Antibody with paraffin-embedded human brain tissue . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Flow cytometric analysis of HepG2 cells using CDH4 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.

CDH4 Antibody

CATALOG NUMBER: 57-420

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This CDH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-203 amino acids from the N-terminal region of human CDH4.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
100 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDH4
Additional Names:
Cadherin-4, Retinal cadherin, R-CAD, R-cadherin, CDH4
Protein Accession Number:
P55283
Protein Gi Number:
81175161
Ncbi Gene Id Number:
1002
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CDH4 is a classical cadherin from the cadherin superfamily. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization.
Background Reference 1:
Miotto,E., Cancer Res. 64 (22), 8156-8159 (2004)
Background Reference 2:
Johnson,E., J. Biol. Chem. 279 (30), 31041-31049 (2004)
Background Reference 3:
Kitagawa,M., Biochem. Biophys. Res. Commun. 271 (2), 358-363 (2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-420

List Size:
400 ul

List Price:

$495.00

Datasheet
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