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Western blot analysis of anti-CDH7 Pab in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with CDH7 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of NCI-H292 cells using Cadherin 7 (CDH7) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of anti-CDH7 Pab in K562 cell line lysates (35ug/lane)

CDH7 Antibody

CATALOG NUMBER: 57-937

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse
Immunogen:
This CDH7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-84 amino acids from the N-terminal region of human CDH7.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
87 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDH7
Additional Names:
Cadherin-7, CDH7, CDH7L1
Protein Accession Number:
Q9ULB5
Protein Gi Number:
296434420
Ncbi Gene Id Number:
1005
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CDH7 is a type II classical cadherin from the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Cadherins mediate cell-cell binding in a homophilic manner, contributing to the sorting of heterogeneous cell types and the maintenance of orderly structures.
Background Reference 1:
Moore,R., Oncogene 23 (40), 6726-6735 (2004)
Background Reference 2:
Kools,P., Genomics 68 (3), 283-295 (2000)
Background Reference 3:
Shimoyama,Y., Biochem. J. 349 (PT 1), 159-167 (2000)
Background Reference 4:
Kremmidiotis,G., Genomics 49 (3), 467-471 (1998)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-937

List Size:
400 ul

List Price:

$495.00

Datasheet
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