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Western blot analysis of extracts of HeLa cells, using CDK2 antibody (18-648).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of HeLa cells, using CDK2 antibody (18-648).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

CDK2 Antibody

CATALOG NUMBER: 18-648

Clonality:
Polyclonal
Tested Applications:
ICC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide of human CDK2
Conjugate:
Unconjugated
Tested Applications:
ICC, IP, WB
Application Note:
WB: 1:500 - 1:2000
ICC: 1:50 - 1:100
IP: 1:20 - 1:50
Positive Control 1:
HeLa
Predicted Molecular Weight:
Observed: 33kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CDK2
Additional Names:
CDK2, CDKN2, p33(CDK2)
Ncbi Gene Id Number:
1017
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-648

List Size:
50 uL

List Price:

$365.00

Out of stock

Datasheet
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