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Western blot analysis of anti-CDK2 Antibody in Jurkat cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CDK2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of jurkat cells using CDK2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of anti-CDK2 Antibody in Jurkat cell line lysates (35ug/lane)

CDK2 Antibody

CATALOG NUMBER: 62-964

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Hamster, Mouse, Rat, Xenopus
Immunogen:
This CDK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-260 amino acids from the C-terminal region of human CDK2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
34 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDK2
Additional Names:
Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase, CDK2, CDKN2
Protein Accession Number:
P24941
Protein Gi Number:
116051
Ncbi Gene Id Number:
1017
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation.
Background Reference 1:
Moshinsky, D.J., et al., Biochem. Biophys. Res. Commun. 310(3):1026-1031 (2003).
Background Reference 2:
Chow, J.P., et al., J. Biol. Chem. 278(42):40815-40828 (2003).
Background Reference 3:
O'Nions, J., et al., Oncogene 22(46):7181-7191 (2003).
Background Reference 4:
Yun, J., et al., J. Biol. Chem. 278(38):36966-36972 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-964

List Size:
400 ul

List Price:

$495.00

Datasheet
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