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Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with starvation (lane 2) using CDK6 (phospho-Tyr24).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDK6 (Phospho-Tyr24).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using CDK6 (phospho-Tyr24).
Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with starvation (lane 2) using CDK6 (phospho-Tyr24).

CDK6 (phospho Tyr24) Antibody

CATALOG NUMBER: 79-249

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
CDK6 (phospho-Tyr24) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 24 (G-A-Y (p) -G-K) derived from Human CDK6.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of CDK6 only when phosphorylated at tyrosine 24.
Predicted Molecular Weight:
36 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CDK6
Additional Names:
PLSTIRE, CREB2, CREBP1, Serine/threonine-protein kinase PLSTIRE
Protein Accession Number:
NP_001138778.1
Protein Gi Number:
223718134
Ncbi Gene Id Number:
1021
Background:
Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins.
Background Reference 1:
Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-8659. Pestell, R. G. et al. (1999) Endocr. Rev. 20, 501-534. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-1625.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-249

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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