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Western blot analysis of CDK8 using rabbit polyclonal CDK8 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDK8 gene.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CDK8 using rabbit polyclonal CDK8 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDK8 gene.

CDK8 Antibody

CATALOG NUMBER: 56-151

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Mouse, Xenopus
Immunogen:
This CDK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-277 amino acids from the Central region of human CDK8.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
53 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDK8
Additional Names:
Cyclin-dependent kinase 8, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Protein kinase K35, CDK8
Protein Accession Number:
P49336
Protein Gi Number:
1345718
Ncbi Gene Id Number:
1024
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of thecyclin-dependent protein kinase (CDK) family. CDK family membersare highly similar to the gene products of Saccharomyces cerevisiaecdc28, and Schizosaccharomyces pombe cdc2, and are known to beimportant regulators of cell cycle progression. This kinase and itsregulatory subunit cyclin C are components of the RNA polymerase IIholoenzyme complex, which phosphorylates the carboxy-terminaldomain (CTD) of the largest subunit of RNA polymerase II. Thiskinase has also been shown to regulate transcription by targetingthe CDK7/cyclin H subunits of the general transcription initiationfactor IIH (TFIIH), thus providing a link between the'Mediator-like' protein complexes and the basal transcriptionmachinery.
Background Reference 1:
Seo, J.O., et al. Oncol. Rep. 24(1):285-291(2010)
Background Reference 2:
Tsutsui, T., et al. Seikagaku 82(3):191-199(2010)
Background Reference 3:
Chattopadhyay, I., et al. Mutat. Res. 696(2):130-138(2010)
Background Reference 4:
Donner, A.J., et al. Nat. Struct. Mol. Biol. 17(2):194-201(2010)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-151

List Size:
400 ul

List Price:

$495.00

Datasheet
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