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Western blot analysis of lysates from HeLa cells using CDKN1C/p57 Kip2 Rabbit mAb at 1:2000 dilution incubated overnight at 4℃. HeLa cells were treated with Dexamethasone (500 nM) at 37℃ for 16hrs . | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.
Western blot analysis of lysates from HeLa cells using CDKN1C/p57 Kip2 Rabbit mAb at 1:2000 dilution incubated overnight at 4℃. HeLa cells were treated with Dexamethasone (500 nM) at 37℃ for 16hrs . | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.

CDKN1C/p57 Kip2 Rabbit Recombinant Antibody (84-183)

CATALOG NUMBER: 84-183

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:2000 - 1:8000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
HeLa treated with Dexamethasone
Predicted Molecular Weight:
32kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CDKN1C
Additional Names:
BWS; WBS; p57; BWCR; KIP2; p57Kip2; CDKN1C/p57 Kip2
Protein Accession Number:
P49918
Ncbi Gene Id Number:
1028
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-183

List Size:
100 uL

List Price:

$477.00

Datasheet
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