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42-131 (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-131 (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CDKN2A Antibody

CATALOG NUMBER: 42-131

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-ILRKKGRLPGEFSE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in lysates of cell line HeLa (calculated MW of 12.1kDa according to NP_478104.1). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize the pancreas isoform (NP_478104.1) only.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CDKN2A
Additional Names:
CDKN2A, cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) , HGNC:1787, ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, TP16, p14, p14ARF, p16, p16INK4, p16INK4a, p19 , CDK4 inhibitor p16-INK4, cell cycle negative regulator beta, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A, p14ARF, cyclin-dependent kinase inhibitor p12, p16INK4a alternatively spliced form, cyclin-dependent kinase inhibitor p16, multiple tumor suppressor 1
Protein Accession Number:
NP_478104.1
Protein Gi Number:
17738298
Ncbi Gene Id Number:
1029
Background Reference 1:
Normand G, Hemmati PG, Verdoodt B, von Haefen C, Wendt J, Guner D, May E, Dorken B, Daniel PT. p14ARF induces G2 cell cycle arrest in p53- and p21-deficient cells by down-regulating p34cdc2 kinase activity. J Biol Chem. 2005 Feb 25;280(8):7118-30. Epub 2004 Dec 6.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-131

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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