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45-033 (1ug/ml) staining of HeLa Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-033 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-033 (1ug/ml) staining of HeLa Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CDKN2A Antibody

CATALOG NUMBER: 45-033

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-HARIDAAEGPSDIPD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 16-18kDa band observed in lysates of the cell lines A431 and HeLa (calculated MW of 16.5kDa according to NP_000068.1). Recommended concentration: 0.1-1ug/ml.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise isoform p16INK4a (NP_000068.1) and isoform p16gamma (NP_001182061.1).
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CDKN2A
Additional Names:
CDKN2A, p16INK4a, cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) , HGNC:1787, ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, TP16, p14, p14ARF, p16, p16INK4, p19 , CDK4 inhibitor p16-INK4, cell cycle negative regulator beta, cyclin-
Protein Accession Number:
NP_000068.1,NP_001182061.1
Protein Gi Number:
4502749
Ncbi Gene Id Number:
1029
Background Reference 1:
Mawrin C, Kirches E, Schneider-Stock R, Boltze C, Vorwerk CK, von Mawrin A, Kirches E, Schneider-Stock R, Boltze C, Vorwerk CK, von Mawrin A, Kirches E, Schneider-Stock R, Boltze C, Vorwerk CK, von Mawrin A, Kirches E, Schneider-Stock R, Boltze C, Vorwerk Alterations of cell cycle regulators in gliomatosis cerebri. J Neurooncol. 2005 Apr;72(2):115-22.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-033

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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