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Western blot analysis of CDKN2B using rabbit polyclonal CDKN2B Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDKN2B gene.
CDKN2B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of CDKN2B Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CDKN2B using rabbit polyclonal CDKN2B Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDKN2B gene.

CDKN2B Antibody

CATALOG NUMBER: 56-550

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CDKN2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the C-terminal region of human CDKN2B.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
15 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDKN2B
Additional Names:
Cyclin-dependent kinase 4 inhibitor B, Multiple tumor suppressor 2, MTS-2, p14-INK4b, p15-INK4b, p15INK4B, CDKN2B, MTS2
Protein Accession Number:
P42772
Protein Gi Number:
1168869
Ncbi Gene Id Number:
1030
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene lies adjacent to the tumor suppressor geneCDKN2A in a region that is frequently mutated and deleted in a widevariety of tumors. This gene encodes a cyclin-dependent kinaseinhibitor, which forms a complex with CDK4 or CDK6, and preventsthe activation of the CDK kinases, thus the encoded proteinfunctions as a cell growth regulator that controls cell cycle G1progression. The expression of this gene was found to bedramatically induced by TGF beta, which suggested its role in theTGF beta induced growth inhibition. Two alternatively splicedtranscript variants of this gene, which encode distinct proteins,have been reported.
Background Reference 1:
Camacho, C.V., et al. Carcinogenesis 31(10):1889-1896(2010)
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
Pechlivanis, S., et al. Arterioscler. Thromb. Vasc. Biol. 30(9):1867-1872(2010)
Background Reference 4:
Heni, M., et al. Diabetes (2010) In press :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-550

List Size:
400 ul

List Price:

$495.00

Datasheet
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