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Antibody is used in Western blot to detect CDX1 in HL60 cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Antibody is used in Western blot to detect CDX1 in HL60 cell lysate.

CDX1 Antibody

CATALOG NUMBER: 62-134

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-265 amino acids from the C-terminal region of human CDX1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDX1
Additional Names:
Homeobox protein CDX-1, Caudal-type homeobox protein 1, CDX1
Protein Accession Number:
P47902
Protein Gi Number:
116241291
Ncbi Gene Id Number:
1044
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded DNA-binding protein regulates intestine-specific gene expression and enterocyte differentiation. It has been shown to induce expression of the intestinal alkaline phosphatase gene, and inhibit beta-catenin/T-cell factor transcriptional activity.
Background Reference 1:
Domon-Dell, C., et al., Oncogene 22(39):7913-7921 (2003).
Background Reference 2:
Suh, E.R., et al., J. Biol. Chem. 277(39):35795-35800 (2002).
Background Reference 3:
Mizoshita, T., et al., Gastric Cancer 4(4):185-191 (2001).
Background Reference 4:
Mallo, G.V., et al., Int. J. Cancer 74(1):35-44 (1997).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-134

List Size:
400 ul

List Price:

$495.00

Datasheet
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