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45-034 (1ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-034 (5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-034 (1ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

CDX2 Antibody

CATALOG NUMBER: 45-034

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: EQLSPGGQRRNLCE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 35kDa band observed in Human Colon lysates (calculated MW of 33.5kDa according to NP_001256.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 5ug/ml.
Specificity:
No cross-reactivity expected with CDX1 and 4
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CDX2
Additional Names:
CDX2, caudal type homeo box transcription factor 2 , HGNC:1806, CDX-3, CDX3
Protein Accession Number:
NP_001256.1
Protein Gi Number:
4502765
Ncbi Gene Id Number:
1045
Background Reference 1:
Wells JM, Melton DA. Vertebrate endoderm development. Annu Rev Cell Dev Biol. 1999;15:393-410. Review.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-034

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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