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IHC testing of FFPE human colon carcinoma with CDX2 antibody (clone CDX2/1690). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.
IHC testing of FFPE human colon carcinoma with CDX2 antibody (clone CDX2/1690). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free CDX2 Antibody (clone CDX2/1690). Confirmation of Integrity and Purity of the Antibody.
IHC testing of FFPE human colon carcinoma with CDX2 antibody (clone CDX2/1690). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

CDX2 Antibody [CDX2/1690]

CATALOG NUMBER: 33-767

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Amino acids 150-249 from the human protein were used as the immunogen for this CDX2 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CDX2 antibody to be titered up or down for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CDX2
Additional Names:
CDX2 Antibody: CDX3, CDX-3, CDX3, Homeobox protein CDX-2
Ncbi Gene Id Number:
1045
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoids and is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma. However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-767

List Size:
100 ug

List Price:

$626.00

Datasheet
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