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Western blot analysis of CEACAM antibody in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma with CEACAM Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HepG2 cells using CEACAM Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CEACAM antibody in HepG2 cell line lysates (35ug/lane)

CEACAM Antibody

CATALOG NUMBER: 62-855

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CEACAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-77 amino acids from the N-terminal region of human CEACAM.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CEACAM1
Additional Names:
Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein 1, BGP-1, CD66a, CEACAM1, BGP, BGP1
Protein Accession Number:
P13688
Protein Gi Number:
399116
Ncbi Gene Id Number:
634
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CEACAM is a member of the carcinoembryonic antigen (CEA) family, which belongs to the immunoglobulin superfamily. The protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses.
Background Reference 1:
Gambichler,T., Am. J. Clin. Pathol. 131 (6), 782-787 (2009)
Background Reference 2:
Li,C., Exp. Cell Res. 315 (7), 1225-1233 (2009)
Background Reference 3:
Nouvion,A.L., Med Sci (Paris) 25 (3), 247-252 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-855

List Size:
400 ul

List Price:

$495.00

Datasheet
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