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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

CEBPA Antibody

CATALOG NUMBER: 31-223

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CEBPA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
CEBPA antibody can be used for detection of CEBPA by ELISA at 1:312500. CEBPA antibody can be used for detection of CEBPA by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
38 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store CEBPA antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
CEBPA
Additional Names:
CEBPA, CEBP, C/EBP-alpha
Protein Accession Number:
NP_004355
Protein Gi Number:
28872794
Ncbi Gene Id Number:
1050
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CEBPA is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. It has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.
Background Reference 1:
Zhu, Q.S., et al., (2004) J. Biol. Chem. 279 (29), 29902-29910.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-223

List Size:
100 ul

List Price:

$519.00

Datasheet
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