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Western blot analysis of CEBPA Antibody in mouse liver tissue lysates (35ug/lane)
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CEBPA Antibody in mouse liver tissue lysates (35ug/lane)

CEBPA Antibody

CATALOG NUMBER: 63-967

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This CEBPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-330 amino acids from the C-terminal region of human CEBPA.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
38 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CEBPA
Additional Names:
CCAAT/enhancer-binding protein alpha, C/EBP alpha, CEBPA
Protein Accession Number:
P49715
Protein Gi Number:
166898082
Ncbi Gene Id Number:
1050
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CEBPA is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. This protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.
Background Reference 1:
Twu, Y.C., et al. Blood 115(12):2491-2499(2010)
Background Reference 2:
Nanri, T., et al. Genes Chromosomes Cancer 49(3):237-241(2010)
Background Reference 3:
Burnett, A.K., et al. Blood 115(5):948-956(2010)
Background Reference 4:
Dufour, A., et al. J. Clin. Oncol. 28(4):570-577(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-967

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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