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43-353 (1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-353 (1ug/ml) staining of Human Skin (A) and Pig Lung (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-353 (1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CEBPB Antibody

CATALOG NUMBER: 43-353

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-DHERAIDFSPYLEP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Human Skin and Pig Lung lysates, and in lysates of cell line NIH3T3 (calculated MW of 36.1kDa according to Human NP_005185.2, 31.5kDa according to Mouse NP_034013.1 and 36.2kDa according to Pig NP_001186818.1). Recommended concentration: 1-3ug/ml.
Predicted Molecular Weight:
Approx 35kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CEBPB
Additional Names:
CEBPB, CCAAT/enhancer binding protein (C/EBP), beta, C/EBP-beta, CRP2, IL6DBP, LAP, NF-IL6, TCF5, CCAAT/enhancer-binding protein beta, TCF-5, interleukin 6-dependent DNA-binding protein, liver-enriched transcriptional activator protein, nuclear factor NF-IL6, nuclear factor of interleukin 6, transcription factor 5
Protein Accession Number:
NP_005185.2
Protein Gi Number:
28872796
Ncbi Gene Id Number:
1051
Background Reference 1:
Canettieri G, Santaguida MG, Antonucci L, Della Guardia M, Franchi A, Coni S, Gulino A, Centanni M.CCAAT/enhancer-binding proteins are key regulators of human type two deiodinase expression in a placenta cell line.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-353

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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