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Western blot analysis of CEBPD in rat spleen tissue lysate with CEBPD antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of CEBPD in human spleen tissue with CEBPD antibody at 20 μg/mL.
Western blot analysis of CEBPD in rat spleen tissue lysate with CEBPD antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

CEBPD Antibody

CATALOG NUMBER: 7077

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Sheep: (100%)
Immunogen:
Rabbit polyclonal CEBPD antibody was raised against an 18 amino acid peptide near the carboxy terminus of human CEBPD.
The immunogen is located within amino acids 170 - 220 of CEBPD.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
CEBPD antibody can be used for detection of CEBPD by Western blot at 0.5 - 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Multiple transcript variants encoding different isoforms have been found for this gene.
Positive Control 1:
Cat. No. 1466 - Rat Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
30 kDa
Purification:
CEBPD Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CEBPD Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CEBPD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CEBPD
Additional Names:
CEBPD Antibody: CELF, CRP3, C/EBP-delta, NF-IL6-betaNuclear factor NF-IL6-beta, NF-IL6-beta
Protein Accession Number:
NP_005186
Protein Gi Number:
28872798
Ncbi Gene Id Number:
1052
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CEBPD Antibody: CCAAT enhancer binding proteins (CEBPs) are a family of transcription factors that all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. At least six members of the family have been isolated and characterized to date (CEBP alpha to CEBP zeta). CEBPD is a leucine zipper (LZ) DNA-binding protein that regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. CEBPD is an important transcriptional activator in the regulation of genes involved in immune and inflammatory responses and has been reported to possess many tumor suppressor-like properties.
Background Reference 1:
Williams SC, Cantwell CA, Johnson PF, et al. A family of C/EBP-related proteins capable of forming covalently linked leucine zipper dimers in vitro. Genes Dev.1991; 5:1553-67.
Background Reference 2:
Umek RM, Friedman AD, and McKnight SL. CCAAT-enhancer binding protein: a component of a differentiation switch. Science 1991; 251:288-92.
Background Reference 3:
Ramji DP and Foka P. CCAAT/enhancer-binding proteins: structure, function and regulation. Biochem. J. 2002; 365:561-75.
Background Reference 4:
Johnson PF, Landschulz WH, Graves BJ, et al. Identification of a rat liver nuclear protein that binds to the enhancer core element of three animal viruses. Genes Dev. 1987; 1:133-46.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7077

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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