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Western blot analysis of extracts of various cell lines, using CEBPG antibody (22-831) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using CEBPG antibody (22-831).
Western blot analysis of extracts of various cell lines, using CEBPG antibody (22-831) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

CEBPG Antibody

CATALOG NUMBER: 22-831

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human CEBPG (NP_001797.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
A-549
Positive Control 2:
HT-29
Positive Control 3:
Mouse liver
Positive Control 4:
Mouse testis
Positive Control 5:
Mouse lung
Predicted Molecular Weight:
Observed: 16kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CEBPG
Additional Names:
CEBPG, GPE1BP, IG/EBP-1
Ncbi Gene Id Number:
1054
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein: a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. The C/EBP family consist of several related proteins, C/EBP alpha, C/EBP beta, C/EBP gamma, and C/EBP delta, that form homodimers and that form heterodimers with each other. CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Two transcript variants encoding the same protein have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-831

List Size:
100 uL

List Price:

$436.00

Datasheet
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