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Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

CELA1 Antibody

CATALOG NUMBER: 59-436

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CELA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the N-terminal region of human CELA1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CELA1
Additional Names:
Chymotrypsin-like elastase family member 1, Elastase-1, Pancreatic elastase 1, CELA1, ELA1
Protein Accession Number:
Q9UNI1
Protein Gi Number:
62298049
Ncbi Gene Id Number:
1990
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Elastases form a subfamily of serine proteases thathydrolyze many proteins in addition to elastin. Humans have sixelastase genes which encode the structurally similar proteinselastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases,pancreatic elastase 1 is not expressed in the pancreas. To date,elastase 1 expression has only been detected in skin keratinocytes.Clinical literature that describes human elastase 1 activity in thepancreas or fecal material is actually referring tochymotrypsin-like elastase family, member 3B.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010)
Background Reference 4:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-436

List Size:
400 ul

List Price:

$495.00

Datasheet
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