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Western blot analysis in Jurkat cell line lysates (35ug/lane).
CELA3A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

CELA3A Antibody

CATALOG NUMBER: 57-763

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CELA3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-186 amino acids from the Central region of human CELA3A.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CELA3A
Additional Names:
Chymotrypsin-like elastase family member 3A, Elastase IIIA, Elastase-3A, Protease E, CELA3A, ELA3, ELA3A
Protein Accession Number:
P09093
Protein Gi Number:
288558842
Ncbi Gene Id Number:
10136
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Elastases form a subfamily of serine proteases thathydrolyze many proteins in addition to elastin. Humans have sixelastase genes which encode the structurally similar proteinselastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase3A has little elastolytic activity. Like most of the humanelastases, elastase 3A is secreted from the pancreas as a zymogenand, like other serine proteases such as trypsin, chymotrypsin andkallikrein, it has a digestive function in the intestine. Elastase3A preferentially cleaves proteins after alanine residues. Elastase3A may also function in the intestinal transport and metabolism ofcholesterol. Both elastase 3A and elastase 3B have been referred toas protease E and as elastase 1.
Background Reference 1:
Shimada, S., et al. Int. J. Mol. Med. 10(5):599-603(2002)
Background Reference 2:
Shirasu, Y., et al. J. Biochem. 104(2):259-264(1988)
Background Reference 3:
Tani, T., et al. J. Biol. Chem. 263(3):1231-1239(1988)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-763

List Size:
400 ul

List Price:

$495.00

Datasheet
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