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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
CELA3B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

CELA3B Antibody

CATALOG NUMBER: 57-258

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CELA3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human CELA3B.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CELA3B
Additional Names:
Chymotrypsin-like elastase family member 3B, Elastase IIIB, Elastase-3B, Protease E, CELA3B, ELA3B
Protein Accession Number:
P08861
Protein Gi Number:
317373457
Ncbi Gene Id Number:
23436
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Elastases form a subfamily of serine proteases thathydrolyze many proteins in addition to elastin. Humans have sixelastase genes which encode the structurally similar proteinselastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase3B has little elastolytic activity. Like most of the humanelastases, elastase 3B is secreted from the pancreas as a zymogenand, like other serine proteases such as trypsin, chymotrypsin andkallikrein, it has a digestive function in the intestine. Elastase3B preferentially cleaves proteins after alanine residues. Elastase3B may also function in the intestinal transport and metabolism ofcholesterol. Both elastase 3A and elastase 3B have been referred toas protease E and as elastase 1, and excretion of this protein infecal material is frequently used as a measure of pancreaticfunction in clinical assays.
Background Reference 1:
Gao, J., et al. Oncol. Rep. 23(6):1683-1692(2010)
Background Reference 2:
Teichmann, J., et al. Eur. J. Med. Res. 13(12):563-567(2008)
Background Reference 3:
Hahn, J.U., et al. Pancreas 36(3):274-278(2008)
Background Reference 4:
Mann, S.T., et al. Eur. J. Med. Res. 13(2):68-72(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-258

List Size:
400 ul

List Price:

$495.00

Datasheet
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