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Antibody used in IHC on Human Skin.
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.
Antibody used in IHC on Human Skin.

CENPA Antibody

CATALOG NUMBER: 30-143

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CENPA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
CENPA antibody can be used for detection of CENPA by ELISA at 1:1562500. CENPA antibody can be used for detection of CENPA by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
16 kDa, 13 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store CENPA antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
CENPA
Additional Names:
CENPA, CenH3, CENP-A
Protein Accession Number:
NP_001800
Protein Gi Number:
4502775
Ncbi Gene Id Number:
1058
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA is a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
Background Reference 1:
Black, B.E., (2004) Nature 430 (6999), 578-582.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-143

List Size:
100 ul

List Price:

$473.00

Datasheet
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