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Antibody used in IF on DNA/ACA.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in IF on DNA/ACA.

CENPM Antibody

CATALOG NUMBER: 26-125

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CENPM.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
CENPM antibody can be used for detection of CENPM by ELISA at 1:312500. CENPM antibody can be used for detection of CENPM by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
20 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store CENPM antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
CENPM
Additional Names:
CENPM, C22orf18, CENP-M, MGC861, PANE1, bK250D10.2
Protein Accession Number:
NP_076958
Protein Gi Number:
13129022
Ncbi Gene Id Number:
79019
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA replaces histone H3. CENPM is an additional factor required for centromere assembly.The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).
Background Reference 1:
Izuta, H., (2006) Genes Cells 11 (6), 673-684.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-125

List Size:
100 ul

List Price:

$519.00

Datasheet
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