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Western blot analysis of CENPN Antibody in MCF-7 cell line lysates (35ug/lane)
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CENPN Antibody in MCF-7 cell line lysates (35ug/lane)

CENPN Antibody

CATALOG NUMBER: 61-708

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CENPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 311-339 amino acids from the C-terminal region of human CENPN.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
40 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CENPN
Additional Names:
Centromere protein N, CENP-N, Interphase centromere complex protein 32, CENPN, C16orf60, ICEN32
Protein Accession Number:
Q96H22
Protein Gi Number:
308153423
Ncbi Gene Id Number:
55839
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPN is an additional factor required for centromere assembly.
Background Reference 1:
Carroll, C.W., et al. Nat. Cell Biol. 11(7):896-902(2009)
Background Reference 2:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 3:
Izuta, H., et al. Genes Cells 11(6):673-684(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-708

List Size:
400 ul

List Price:

$495.00

Datasheet
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