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Western blot analysis of CENPO in mouse kidney tissue lysate with CENPO antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of CENPO in mouse kidney tissue lysate with CENPO antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

CENPO Antibody

CATALOG NUMBER: 5433

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
CENPO antibody was raised against a 16 amino acid synthetic peptide from near the center of human CENPO.
The immunogen is located within amino acids 170 - 220 of CENPO.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
CENPO antibody can be used for detection of CENPO by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Purification:
CENPO Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CENPO Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CENPO antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CENPO
Additional Names:
CENPO Antibody: CENP-O, MCM21R, ICEN-36, ICEN36, Centromere protein O, Interphase centromere complex protein 36, CENP-O
Protein Accession Number:
NP_077298
Protein Gi Number:
13236565
Ncbi Gene Id Number:
79172
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CENPO Antibody: Accurate chromosome segregation during mitosis requires a kinetochore to assemble correctly at the centromere of each chromatid and form a dynamic interface with the microtubules of the mitotic spindle. The kinetochore assembly includes the multisubunit CENP-H/I complex which can be divided into three functional classes based on phenotype analysis. One of these classes is referred to as the CENP-O class of proteins of which CENPO is the exemplar. These proteins form a stable complex with each other and are required for proper kinetochore function and for recovery from mitotic spindle damage. CENPO has also been shown to be an auto-antigen in a small population of anti-centromere antibodies- (ACA-) positive patients with sclerodoma.
Background Reference 1:
Fukagawa T. Assembly of kinetochore in vertebrate cells. Exp. Cell Res.2004; 296:21-7.
Background Reference 2:
Okada M, Cheeseman IM, Hori T, et al. The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres. Nat. Cell Biol.2006; 8:446-57.
Background Reference 3:
Hori T, Okada M, Maenaka K, et al. CENP-O class proteins form a stable complex and are required for proper kinetochore function. Mol. Biol. Cell2008; 19:843-54.
Background Reference 4:
Saito A, Muro Y, Sugiura K, et al. CENP-O, a protein localized at the centromere throughout the cell cycle, is a novel target antigen in systemic sclerosis. J. Rheumatol.2009; 36:781-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5433

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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