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45-390 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-390 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

CENTA2 Antibody

CATALOG NUMBER: 45-390

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-RLTASTESGRSSR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments in Human Brain and Heart lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Placenta shows trong staining of cytoplasm in syncytiotrophoblasts.. Recommended concentration, 5-10ug/ml.
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ADAP2
Additional Names:
ADAP2, CENTA2, centaurin, alpha 2, HSA272195, centaurin-alpha 2 protein, ArfGAP with dual PH domains 2, cent-b, centaurin beta
Protein Accession Number:
NP_060874.1
Protein Gi Number:
8923763
Ncbi Gene Id Number:
55803
Background Reference 1:
Jenne DE, Tinschert S, Stegmann E, Reimann H, N]urnberg P, Horn D, Naumann I, Buske A, Thiel G. A common set of at least 11 functional genes is lost in the majority of NF1 patients with gross deletions. Genomics. 2000 May 15;66(1):93-7.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-390

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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