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Centromere Protein E Antibody

CATALOG NUMBER: 45-392

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: HASSGKDVPECKTQ
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 316kDa according to NP_001804. Therefore we cannot recommend an optimal concentration and the product is Investigative Grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CENPE
Additional Names:
CENPE, CENP-E, centromere protein E (312kD), Centromere autoantigen E (312kD), centromere protein E, 312kDa, KIF10, centromere protein E, kinesin family member 10
Protein Accession Number:
NP_001804.2
Protein Gi Number:
71061468
Ncbi Gene Id Number:
1062
Background Reference 1:
Yen TJ, Compton DA, Wise D, Zinkowski RP, Brinkley BR, Earnshaw WC, Cleveland DW. CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase. EMBO J. 1991 May;10(5):1245-54.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-392

List Size:
0.1 mg

List Price:

$391.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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