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46-640 (0.03ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-640 (2.5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-640 (0.03ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CES1 Antibody

CATALOG NUMBER: 46-640

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-NTQAAQKLKDKE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 55-60kDa band observed in Human Liver and Ileum lysates (calculated MW of 62.6kDa according to NP_001020366.1). In transfected HEK293 transiently expressing CES1 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml.Immunohistochemistry:In paraffin embedded Human Lung shows staining of lymphocytes in the vasculature.. Recommended concentration, 2-3ug/ml.
Specificity:
This antibody is expected to recognise all three reported isoforms (NP_001020366.1; NP_001020365.1; NP_001257.4).
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CES1
Additional Names:
CES1, carboxylesterase 1 (monocyte/macrophage serine esterase 1), ACAT, CEH, CES2, HMSE, HMSE1, MGC117365, PCE-1, SES1, TGH, acyl coenzyme A:cholesterol acyltransferase, carboxylesterase 1, carboxylesterase 2 (liver), cholesteryl ester hydrolase, egasyn, liver carboxylesterase, triacylglycerol hydrolase, monocyte/macrophage serine-esterase 1
Protein Accession Number:
NP_001020366.1,NP_001020365.1,NP_001257.4
Protein Gi Number:
68508967
Ncbi Gene Id Number:
1066
Background Reference 1:
Steinberg GR, Kemp BE, Watt MJ. Adipocyte triglyceride lipase expression in human obesity. Am J Physiol Endocrinol Metab. 2007 Oct;293(4):E958-64. Epub 2007 Jul 3.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-640

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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