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Western blot analysis of extracts of various cell lines, using CETN2 Antibody (19-637) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunofluorescence analysis of A549 cells using CETN2 antibody (19-637).
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using CETN2 antibody (19-637). GreenGFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Western blot analysis of extracts of various cell lines, using CETN2 Antibody (19-637) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

CETN2 Antibody

CATALOG NUMBER: 19-637

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CETN2 (NP_004335.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
U-251MG
Positive Control 2:
SKOV3
Positive Control 3:
HeLa
Positive Control 4:
HepG2
Positive Control 5:
A-549
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 20kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CETN2
Additional Names:
CALT, CEN2, centrin-2, caltractin (20kD calcium-binding protein), centrin, EF-hand protein, 2
Ncbi Gene Id Number:
1069
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Caltractin belongs to a family of calcium-binding proteins and is a structural component of the centrosome. The high level of conservation from algae to humans and its association with the centrosome suggested that caltractin plays a fundamental role in the structure and function of the microtubule-organizing center, possibly required for the proper duplication and segregation of the centrosome.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-637

List Size:
100 uL

List Price:

$540.00

Datasheet
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