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Western blot analysis in CEM(lane 1),K562(lane 2) cell line lysates (15ug/lane).
CFHR5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in CEM(lane 1),K562(lane 2) cell line lysates (15ug/lane).

CFHR5 Antibody

CATALOG NUMBER: 62-009

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CFHR5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-231 amino acids from the Central region of human CFHR5.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
64 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CFHR5
Additional Names:
Complement factor H-related protein 5, FHR-5, CFHR5, CFHL5, FHR5
Protein Accession Number:
Q9BXR6
Protein Gi Number:
23396597
Ncbi Gene Id Number:
81494
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CFHR5 is a member of a small complement factor H (CFH)gene cluster on chromosome 1. Each member of this gene familycontains multiple short consensus repeats (SCRs) typical ofregulators of complement activation. The protein encoded by thisgene has nine SCRs with the first two repeats having heparinbinding properties, a region within repeats 5-7 having heparinbinding and C reactive protein binding properties, and theC-terminal repeats being similar to a complement component 3 b(C3b) binding domain. This protein co-localizes with C3, binds C3bin a dose-dependent manner, and is recruited to tissues damaged byC-reactive protein. Allelic variations in this gene have beenassociated, but not causally linked, with two different forms ofkidney disease: membranoproliferative glomerulonephritis type II(MPGNII) and hemolytic uraemic syndrome (HUS). [provided byRefSeq].
Background Reference 1:
McRae, J.L., et al. Genetica 114(2):157-161(2002)
Background Reference 2:
Murphy, B., et al. Am. J. Kidney Dis. 39(1):24-27(2002)
Background Reference 3:
Narkio-Makela, M., et al. Clin. Immunol. 100(1):118-126(2001)
Background Reference 4:
McRae, J.L., et al. J. Biol. Chem. 276(9):6747-6754(2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-009

List Size:
400 ul

List Price:

$495.00

Datasheet
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