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Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of CFL1 Antibody in HL-60 cell line lysates (35ug/lane).
Western blot analysis of CFL1 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)
Western blot analysis of CFL1 using rabbit polyclonal CFL1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CFL1 gene.
Flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

CFL1 Antibody

CATALOG NUMBER: 61-589

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Pig, Rat, Chicken, Xenopus
Immunogen:
This CFL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the Central region of human CFL1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
19 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CFL1
Additional Names:
Cofilin-1, 18 kDa phosphoprotein, p18, Cofilin, non-muscle isoform, CFL1, CFL
Protein Accession Number:
P23528
Protein Gi Number:
116848
Ncbi Gene Id Number:
1072
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner.
Background Reference 1:
Fazal,F., et.al., J. Biol. Chem. 284 (31), 21047-21056 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-589

List Size:
400 ul

List Price:

$495.00

Datasheet
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