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Western blot analysis of CFP Antibody in CEM cell line lysates (35ug/lane)
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CFP Antibody in CEM cell line lysates (35ug/lane)

CFP Antibody

CATALOG NUMBER: 64-199

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CFP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 197-223 amino acids from the Central region of human CFP.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
51 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CFP
Additional Names:
Properdin, Complement factor P, CFP, PFC
Protein Accession Number:
P27918
Protein Gi Number:
464473
Ncbi Gene Id Number:
5199
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections.
Background Reference 1:
Seitsonen, S., et al. Mol. Immunol. 47(6):1334-1336(2010)
Background Reference 2:
Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009)
Background Reference 3:
Wolf-Schnurrbusch, U.E., et al. Retina (Philadelphia, Pa.) 29(7):966-973(2009)
Background Reference 4:
Keslar, K., et al. Transplantation 86(9):1319-1321(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-199

List Size:
400 ul

List Price:

$495.00

Datasheet
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