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IHC testing of FFPE human pancreas with CFTR antibody (clone CFTR1-1).
IHC testing of FFPE mouse kidney with CFTR antibody (clone CFTR1-1).
IHC testing of FFPE human pancreas with CFTR antibody (clone CFTR1-1).

CFTR Antibody [CFTR1-1]

CATALOG NUMBER: 34-037

Clonality:
Monoclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Immunogen:
Human recombinant protein was used as the immunogen for this CFTR antibody.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
Immunofluorescence: 1-2 ug/ml
Western blot: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CFTR antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CFTR
Additional Names:
CFTR Antibody: CF, MRP7, ABC35, ABCC7, CFTR/MRP, TNR-CFTR, dJ760C5.1, Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, CFTR
Ncbi Gene Id Number:
1080
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Cystic fibrosis transmembrane conductance regulator (CFTR) is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (MDR1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-037

List Size:
100 ug

List Price:

$626.00

Datasheet
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