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IHC testing of FFPE human pancreas with CFTR antibody (clone SPM176). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.
IHC testing of FFPE human pancreas with CFTR antibody (clone SPM176). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.

CFTR Antibody [SPM176]

CATALOG NUMBER: 33-630

Clonality:
Monoclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Immunogen:
Human partial recombinant protein was used as the immunogen for this CFTR antibody.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
IF: 0.5-1 ug/ml
Western blot: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CFTR antibody to be titered up or down for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2a
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CFTR
Additional Names:
CFTR Antibody: CF, MRP7, ABC35, ABCC7, CFTR/MRP, TNR-CFTR, dJ760C5.1, Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, CFTR
Ncbi Gene Id Number:
1080
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Cystic fibrosis transmembrane conductance regulator (CFTR) is involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. Can inhibit the chloride channel activity of ANO1. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation. [UniProt]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-630

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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