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Western blot analysis of CHADL in mouse kidney tissue lysate with CHADL antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of CHADL in mouse skeletal muscle tissue with CHADL antibody at 5 μg/mL.
Immunofluorescence of CHADL in mouse skeletal muscle tissue with CHADL antibody at 20 μg/mL.<br><br>Red: CHADL Antibody (8423) <br> Blue: DAPI staining
Immunohistochemistry of CHADL in mouse skeletal muscle tissue with CHADL antibody at 5 μg/mL.
Western blot analysis of CHADL in mouse kidney tissue lysate with CHADL antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

CHADL Antibody

CATALOG NUMBER: 8423

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
CHADL antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human CHADL.
The immunogen is located within the last 50 amino acids of CHADL.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC, WB
Application Note:
CHADL antibody can be used for the detection of CHADL by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
CHADL antibody is human, mouse and rat reactive. CHADL antibody is predicted to not cross-react with Chondroadherin protein.
Positive Control 1:
Cat. No. 1467 - Rat Skeletal Muscle Tissue Lysate
Predicted Molecular Weight:
Predicted: 84 kDa
Observed: 86 kDa
Purification:
CHADL Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CHADL Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CHADL antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
CHADL
Additional Names:
Chondoadherin-like protein, SLRR4B
Protein Accession Number:
NP_612490
Protein Gi Number:
194440719
Ncbi Gene Id Number:
150356
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Chondroadherin-like (CHADL) protein, also known as SLRR4B, is homologous to the Chondroadherin protein and is expressed cartillaginous tissues (1). Like Chondroadherin, CHADL belongs to the small leucine-rich proteoglycan (SLRP) family and class IV subfamily and plays a significant role in the regulation of chondrocyte growth and proliferation (1,2). CHADL is thought to play a negative regualtory role in collagen fibrillogenesis and chondrocyte differentiation (1).
Background Reference 1:
Tillgren V, Ho JC, Onnerfjord P, et al. The novel small leucine-rich protein chondroadherin-like (CHADL) is expressed in cartilage and modulates chondrocyte differentitation. J. Biol. Chem. 2015; 290:918-25.
Background Reference 2:
Neame PJ, Sommarin Y, Boynton RE, et al. The structure of a 38-kDa leucine-rich protein (chondroadherin) isolated from bovine cartilage. J. Biol. Chem. 1994; 269:21547-54.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8423

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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