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45-037 (1:2000) staining of PFA-perfused cryosection of Mouse Forebrain. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method. Data obtained by Drs. Éva Rumpler and Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary.
45-037 (1:2000) staining of PFA-perfused cryosection of Mouse Forebrain. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method. Data obtained by Drs. Éva Rumpler and Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary.

ChAT Antibody

CATALOG NUMBER: 45-037

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-KERARGPTKPSNLD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3kDa according to Mouse NP_034021.1 and Rat NP_001164064.1). Recommended concentration:0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Frozen sections of Mouse Forebrain shows staining of neural cell bodies. Optimal dilution: 1:2000.
Specificity:
This antibody is NOT expected to cross-react to Human CHAT.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Chat
Additional Names:
Chat, choline acetyltransferase , B230380D24Rik
Protein Accession Number:
NP_034021.1
Protein Gi Number:
45476575
Ncbi Gene Id Number:
12647(mouse);432359(rat);
Background Reference 1:
Contestabile A, Fila T, Bartesaghi R, Contestabile A, Ciani E. Choline acetyltransferase activity at different ages in brain of Ts65Dn mice, an animal model for Down's syndrome and related neurodegenerative diseases. J Neurochem. 2006 Apr;97(2):515-26. Epub 2006 Mar 15.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-037

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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