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Western blot analysis of CHAT Antibody in NCI-H460 cell line lysates (35ug/lane)
Western blot analysis of CHAT Antibody in mouse liver tissue lysates (35ug/lane)
Confocal immunofluorescent analysis of CHAT Antibody with NCI-H460 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of CHAT Antibody in NCI-H460 cell line lysates (35ug/lane)

CHAT Antibody

CATALOG NUMBER: 64-014

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This CHAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-128 amino acids from the N-terminal region of human CHAT.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
83 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CHAT
Additional Names:
Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, CHAT
Protein Accession Number:
P28329
Protein Gi Number:
281185509
Ncbi Gene Id Number:
1103
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CHAT is an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease.
Background Reference 1:
Shaffer, J.R., et al. Nutr Metab Cardiovasc Dis (2010) In press
Background Reference 2:
Beck, M., et al. Cell Tissue Res. 338(1):37-51(2009)
Background Reference 3:
Need, A.C., et al. Eur. J. Hum. Genet. 17(7):946-957(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-014

List Size:
400 ul

List Price:

$495.00

Datasheet
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