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Western blot analysis of extracts of various cell lines, using CHD3 antibody (23-968) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using CHD3 antibody (23-968) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.

CHD3 Antibody [4F6B11]

CATALOG NUMBER: 23-968

Clonality:
Monoclonal
Tested Applications:
Flow, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant protein of human CHD3
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Flow: 1:20 - 1:50
Positive Control 1:
293T
Positive Control 2:
HL-60
Positive Control 3:
Jurkat
Positive Control 4:
K-562
Positive Control 5:
A375
Positive Control 6:
LO2
Predicted Molecular Weight:
Observed: 270kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CHD3
Additional Names:
Mi-2a, Mi2-ALPHA, SNIBCPS, ZFH, chromodomain-helicase-DNA-binding protein 3, ATP-dependent helicase CHD3, CHD-3, hZFH, mi-2 autoantigen 240 kDa protein, zinc finger helicase, zinc-finger helicase (Snf2-like)
Ncbi Gene Id Number:
1107
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-968

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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