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Western blot analysis of extracts of various cell lines, using CHD4 antibody (23-969) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using CHD4 antibody (23-969) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

CHD4 Antibody [4D10G6]

CATALOG NUMBER: 23-969

Clonality:
Monoclonal
Tested Applications:
WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant protein of human CHD4
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:1000
Positive Control 1:
293T
Positive Control 2:
HL-60
Positive Control 3:
Jurkat
Positive Control 4:
K-562
Positive Control 5:
A375
Positive Control 6:
LO2
Predicted Molecular Weight:
Observed: 300kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CHD4
Additional Names:
CHD4, ATP-dependent helicase CHD4, CHD-4, Mi-2b, Mi2-BETA
Ncbi Gene Id Number:
1108
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-969

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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