Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of CHD7 in SK-N-SH cell lysate with CHD7 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of CHD7 in mouse brain tissue with CHD7 antibody at 5 μg/mL.
Immunofluorescence of CHD7 in mouse brain tissue with CHD7 antibody at 20 μg/mL.
Western blot analysis of CHD7 in SK-N-SH cell lysate with CHD7 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

CHD7 Antibody

CATALOG NUMBER: 6441

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
CHD7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CHD7.
The immunogen is located within amino acids 670 - 720 of CHD7.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
CHD7 antibody can be used for detection of CHD7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
Multiple isoforms of CHD7 are known to exist.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Purification:
CHD7 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CHD7 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CHD7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CHD7
Additional Names:
CHD7 Antibody: CRG, HH5, IS3, KAL5, KIAA1416, Chromodomain-helicase-DNA-binding protein 7, ATP-dependent helicase CHD7, CHD-7
Protein Accession Number:
Q9P2D1
Protein Gi Number:
54112403
Ncbi Gene Id Number:
55636
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CHD7 Antibody: CHD proteins belong to a superfamily of proteins of ATP-dependent chromatin remodeling enzymes that have a unique combination of functional domains, including two N-terminal chromodomains, a SNF2-like ATPase/helicase domain and a DNA-binding domain. These proteins are thought to play a role in early embryonic development by affecting chromatin structure and gene expression. Mutations in one member of this family, CHD7, result in CHARGE syndrome. It colocalizes with embryonic stem (ES) cell master regulators OCT4/POU5F1, SOX2, and NANOG and is thought to modulate ES-specific gene transcription. Together with SOX2, CHD7 has been suggested to also regulate several human disease genes.
Background Reference 1:
Woodage T, Basrai MA, Baxevanis AD, et al. Characterization of the CHD family of proteins. Proc. Natl. Acad. Sci. USA 1997; 94:11472-7.
Background Reference 2:
Cavalli G and Paro R. Chromo-domain proteins: linking chromatin structure to epigenetic regulation. Curr. Opin. Cell Biol. 1998; 10:354-60.
Background Reference 3:
Vissers LE, van Ravenswaaj CM, Admiraal R, et al. Mutations in a new member of the chromodomain gene family cause CHARGE syndrome. Nat. Genet. 2004; 36:955-7.
Background Reference 4:
Schnetz MP, Handoko L, Akhtar-Zaidi B, et al. CHD7 targets active gene enhancer elements to modulate ES cell-specific gene expression. PLoS Genet. 2010; 6:31001023.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6441

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369