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Western blot analysis of extracts of various cell lines, using CHD9 antibody (14-279) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using CHD9 antibody (14-279) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

CHD9 Antibody

CATALOG NUMBER: 14-279

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 85-190 of human CHD9 (NP_079410.4).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
U-87MG
Positive Control 2:
HeLa
Positive Control 3:
Jurkat
Positive Control 4:
A-549
Positive Control 5:
293T
Positive Control 6:
K-562
Predicted Molecular Weight:
Observed: 300kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CHD9
Additional Names:
AD013, CHD-9, CReMM, KISH2, PRIC32chromodomain-helicase-DNA-binding protein 9, ATP-dependent helicase CHD9, PPAR-alpha-interacting complex protein 320 kDa, PPAR{gamma}-interacting cofactor 320 kDa, chromatin remodeling factor CHROM1, chromatin-related mesenchymal modulator, ciprofibrate bound protein p24kismet homolog 2, peroxisomal proliferator-activated receptor A-interacting complex 320 kDa protein, proteinx0008
Ncbi Gene Id Number:
80205
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-279

List Size:
100 uL

List Price:

$436.00

Datasheet
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