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Antibody used in IHC on Human kidney.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in IHC on Human kidney.

CHEK1 Antibody

CATALOG NUMBER: 27-641

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHEK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
CHEK1 antibody can be used for detection of CHEK1 by ELISA at 1:312500. CHEK1 antibody can be used for detection of CHEK1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
54 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store CHEK1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
CHEK1
Additional Names:
CHEK1, CHK1
Protein Accession Number:
NP_001265
Protein Gi Number:
166295192
Ncbi Gene Id Number:
1111
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CHEK1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. Its expression is dispensable for somatic cell death and critical for sustaining G2 DNA damage checkpoint.
Background Reference 1:
Kramer, A., et al., (2004) Nat. Cell Biol. 6 (9), 884-891.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-641

List Size:
100 ul

List Price:

$519.00

Datasheet
Shipping Info

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