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43-391 (2ug/ml) staining of Rat Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-391 (2ug/ml) staining of Rat Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CHEK1 Antibody

CATALOG NUMBER: 43-391

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Cow
Immunogen:
The immunogen for this antibody is: DQPSDSCQEYSD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Rat Thymus lysates (calculated MW of 54.4kDa according to Rat NP_536325.1). Recommended concentration: 1-3ug/ml.
Predicted Molecular Weight:
Approx 50kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CHEK1
Additional Names:
CHEK1 , CHK1 checkpoint homolog (S. pombe) , CHK1, CHK1 checkpoint homolog , Checkpoint, S. pombe, homolog of, 1
Protein Accession Number:
NP_001265.1
Protein Gi Number:
166295192
Ncbi Gene Id Number:
1111
Background Reference 1:
Kim MA, Kim HJ, Brown AL, Lee MY, Bae YS, Park JI, Kwak JY, Chung JH, Yun J.Identification of novel substrates for human checkpoint kinase Chk1 and Chk2 through genome-wide screening using a consensus Chk phosphorylation motif.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-391

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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