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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in IHC on Human Breast.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

CHFR Antibody

CATALOG NUMBER: 25-840

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHFR.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
CHFR antibody can be used for detection of CHFR by ELISA at 1:312500. CHFR antibody can be used for detection of CHFR by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
69 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store CHFR antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
CHFR
Additional Names:
CHFR, FLJ10796, FLJ33629, RNF116, RNF196
Protein Accession Number:
NP_060693
Protein Gi Number:
239048907
Ncbi Gene Id Number:
55743
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CHFR is an E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome moves apart from each other along the periphery of the nucleus. CHFR probably promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. This suggests that it may be involved in signaling the presence of mitotic stress caused by microtubule poisons.
Background Reference 1:
Kang, H.C., Cancer Lett. 260 (1-2), 170-179 (2008).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-840

List Size:
100 ul

List Price:

$519.00

Datasheet
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