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45-397 (2.5ug/ml) staining of paraffin embedded human brain (cerebellum). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-397 (2.5ug/ml) staining of paraffin embedded human brain (cerebellum). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

CHFR Antibody

CATALOG NUMBER: 45-397

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: KFNHICEQTRFKN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 80kDa band in human brain lysates at 0.5ug/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 72kDda according to NP_060693.Immunohistochemistry:In paraffin embedded Human Brain (Cerebellum) shows stained Purkinje cells. Recommended concentration: 2.5ug/ml.
Specificity:
This antibody is expected to recognise all reported isoforms.
Positive Control 1:
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CHFR
Additional Names:
CHFR, checkpoint with forkhead and ring finger domains, FLJ10796, FLJ33629, RNF116, RNF196, E3 ubiquitin-protein ligase CHFR, RING finger protein 196
Protein Accession Number:
NP_001154816.1,NP_001154817.1,NP_001154818.1,NP_060693.2,NP_001154819.1
Protein Gi Number:
239048907
Ncbi Gene Id Number:
55743
Background Reference 1:
Scolnick DM, Halazonetis TD. Chfr defines a mitotic stress checkpoint that delays entry into metaphase. Nature. 2000 Jul 27;406(6794):430-5.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-397

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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