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Western blot analysis in Hela cell line lysates (35ug/lane).
CHI3L2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Hela cell line lysates (35ug/lane).

CHI3L2 Antibody

CATALOG NUMBER: 56-816

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CHI3L2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-50 amino acids from the N-terminal region of human CHI3L2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
44 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CHI3L2
Additional Names:
Chitinase-3-like protein 2, Chondrocyte protein 39, YKL-39, CHI3L2
Protein Accession Number:
Q15782
Protein Gi Number:
13124005
Ncbi Gene Id Number:
1117
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CHI3L2 may bind glycan structure with high affinity, but not heparin. Has no chitotriosidase activity.
Background Reference 1:
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Background Reference 2:
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Background Reference 3:
Steck, E., et al. Biochem. Biophys. Res. Commun. 299(1):109-115(2002)
Background Reference 4:
Hu, B., et al. J. Biol. Chem. 271(32):19415-19420(1996)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-816

List Size:
400 ul

List Price:

$469.00

Out of stock

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